效率：
Competitive Testing with Cyclooxygenase-2：TFF可省下70%的時間。
規格：
| Minimate EVO Tangential flow Filtration System |
| 裝置包含 |
Includes peristaltic pump, pump head,2 pressure gauges, reservoir,
stir plate, drip tray, and assorted fittings |
| 整體大小 |
30.7 cm W x 48.2 cm D x 20.8 cm H
(12.1 in. W x 19 in. D x 8.2 in. H) |
| 整體重量 |
6.0 kg (13.2 lbs.) |
| 最大進口壓力 |
4.1 bar (410 kPa, 60 psi) |
| 操作溫度範圍 |
0 - 50 °C (0 - 106 °F) |
| 循環流速 |
10 - 240 mL/min |
| 最小體積 |
15 mL |
| 樣品槽材質 |
Polysulfone |
| 管線材質 |
PharMedu #16 |
應用於外泌體純化
引用文獻
1. A novel approach to alleviate acetaminophen-induced hepatotoxicity with hybrid balloon flower root-derived exosome-like nanoparticles (BDEs) with silymarin via inhibition of hepatocyte MAPK pathway and apoptosis. Cell Commun Signal. 2024 Jun 18;22(1):334. doi: 10.1186/s12964-024-01700-z.
分離桔梗根部衍生之類外泌體奈米粒子(exosome-like nanoparticles, BDEs) <使用500 kDa濾匣>
2. Extracellular Vesicles, as Drug-Delivery Vehicles, Improve the Biological Activities of Astaxanthin. Antioxidants 2023, 12(2), 473: doi.org/10.3390/antiox12020473
胎牛血清衍生外泌體(FBS-derived EVs) <使用300 kDa濾匣>
3. Comparative analysis of tangential flow filtration and ultracentrifugation, both combined with subsequent size exclusion chromatography, for the isolation of small extracellular vesicles. J Extracell Vesicles. 2022 Sep;11(9):e12266. doi: 10.1002/jev2.12266.
在此篇論文中，明確指出使用300kDa之濾匣可有效分離sEVs。
從子宮頸癌細胞株HeLa, 乳癌細胞株MDA‐MB‐231, 老鼠乳癌細胞EO771及老鼠黑色素癌細胞B16F10 之 細胞培養後之條件培養液(conditioned media)分離之外泌體。
4. Isolation and Characterization of Extracellular Vesicle from Mesenchymal Stem Cells of the Epidural Fat of the Spine. Asian Spine J. 2022 Apr;16(2):153-161. doi: 10.31616/asj.2021.0129. Epub 2021 Sep 1.
從椎管内硬膜外脂肪（epidural fat，EF）的MSC中分離出EVs <使用100 kDa(10nm)濾匣>
5. Functional siRNA Delivery by Extracellular Vesicle–Liposome Hybrid Nanoparticles. Adv Healthc Mater. 2022 Mar;11(5):e2101202. doi: 10.1002/adhm.202101202. Epub 2021 Aug 11.
分離cardiac progenitor cell (CPC)-EVs <使用100 kDa濾匣>
6. Reactive oxygen species-responsive dendritic cell-derived exosomes for rheumatoid arthritis. Acta Biomater. 2021 Jul 1:128:462-473. doi: 10.1016/j.actbio.2021.04.026. Epub 2021 Apr 18.
耐受性樹突狀細胞(tolerogenic DC, tolDC)衍生之exosome <使用300 kDa濾匣>
7. A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA. Nat Commun. 2020 Feb 28;11(1):1113. doi: 10.1038/s41467-020-14977-8.
分離MDA-MB-231 sgRNA+細胞之EVs <使用100 kDa濾匣>
8. Microvesicles from brain-extract—treated mesenchymal stem cells improve neurological functions in a rat model of ischemic stroke. Sci Rep. 2016 Sep 9:6:33038. doi: 10.1038/srep33038.
分離human MSCs之microvesicles (MVs) <使用100 kDa濾匣>
Google patent
WO2018004145A1 - Anti-inflammatory composition containing yeast-derived extracellular vesicle
分離酵母菌衍生之外泌體(yeast derived exosomes) <使用100 kDa濾匣>
訂購資訊
| 貨號 |
品名 |
內含物 |
| OAPMPUNV |
Minimate EVO TFF system package (kit) |
Peristaltic pump, pump head, 2 pressure gauges,
reservoir, stir plate, drip tray, and assorted fittings |
| 97014 |
Minimate EVO Fittings Kit |
3-way valve for reservoir (2), 1-way valve reservoir lid (2), male luer coupler (1),
male luer lock - 1/8” hose barb (3),male luer lock - 1/8” hose barb elbow (4),
female luer lock - 1/8” hose barb (1), tubing clamps (8), PharMed tubing (2 ft.),
screw clamp for tubing (1), male luer plug (4) |
| 97008 |
Minimate EVO Reservoir Kit |
- |
| 97010 |
Minimate EVO Stir Plate Kit |
- |
| 97011 |
Minimate EVO Pump Drive |
- |
| 97009 |
Minimate EVO Drip Tray Kit |
- |
| 97012 |
Minimate EVO Pump Head |
- |